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pbabe neo gateway erbb2  (Addgene inc)


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    Structured Review

    Addgene inc pbabe neo gateway erbb2
    Pbabe Neo Gateway Erbb2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbabe+neo+gateway+erbb2/pBABE-neo+(Plasmid+%231767)/pmc11245391__41388_2024_3070_MOESM1_ESM-2-35-44
    Average 93 stars, based on 41 article reviews
    pbabe neo gateway erbb2 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Retroviral:

    Article Title:
    Article Snippet: The 7 corresponding 737 bp fragment containing the gene for puromycin-resistance was removed 8 from pBABE-puro-gateway (Plasmid #51070; http://n2t.net/addgene:51070) with the same 9 pair of restriction enzymes creating a 6 141 bp fragment of the vector backbone. .. The insert (1 10 862 bp) encoding Neo was ligated (T4 DNA ligase; Thermo Scientific) with the digested vector 11 (6 141 bp), thereby incorporating the neomycin resistance gene into the pBABE-gateway 12 retroviral vector. pBABE-neo-gateway-ERBB2 was subsequently constructed by Gateway 13 cloning [1] (Addgene). pBABE-puro-gateway-ERBB3 plasmid was constructed by a LR-14 Gateway recombination reaction between the wild-type pDONR221-ERBB3 [2] and the 15 pBABE-puro-gateway using LR clonase II mix (Invitrogen). ..

    Construct:

    Article Title:
    Article Snippet: The 7 corresponding 737 bp fragment containing the gene for puromycin-resistance was removed 8 from pBABE-puro-gateway (Plasmid #51070; http://n2t.net/addgene:51070) with the same 9 pair of restriction enzymes creating a 6 141 bp fragment of the vector backbone. .. The insert (1 10 862 bp) encoding Neo was ligated (T4 DNA ligase; Thermo Scientific) with the digested vector 11 (6 141 bp), thereby incorporating the neomycin resistance gene into the pBABE-gateway 12 retroviral vector. pBABE-neo-gateway-ERBB2 was subsequently constructed by Gateway 13 cloning [1] (Addgene). pBABE-puro-gateway-ERBB3 plasmid was constructed by a LR-14 Gateway recombination reaction between the wild-type pDONR221-ERBB3 [2] and the 15 pBABE-puro-gateway using LR clonase II mix (Invitrogen). ..

    Cloning:

    Article Title:
    Article Snippet: The 7 corresponding 737 bp fragment containing the gene for puromycin-resistance was removed 8 from pBABE-puro-gateway (Plasmid #51070; http://n2t.net/addgene:51070) with the same 9 pair of restriction enzymes creating a 6 141 bp fragment of the vector backbone. .. The insert (1 10 862 bp) encoding Neo was ligated (T4 DNA ligase; Thermo Scientific) with the digested vector 11 (6 141 bp), thereby incorporating the neomycin resistance gene into the pBABE-gateway 12 retroviral vector. pBABE-neo-gateway-ERBB2 was subsequently constructed by Gateway 13 cloning [1] (Addgene). pBABE-puro-gateway-ERBB3 plasmid was constructed by a LR-14 Gateway recombination reaction between the wild-type pDONR221-ERBB3 [2] and the 15 pBABE-puro-gateway using LR clonase II mix (Invitrogen). ..

    Plasmid Preparation:

    Article Title:
    Article Snippet: The 7 corresponding 737 bp fragment containing the gene for puromycin-resistance was removed 8 from pBABE-puro-gateway (Plasmid #51070; http://n2t.net/addgene:51070) with the same 9 pair of restriction enzymes creating a 6 141 bp fragment of the vector backbone. .. The insert (1 10 862 bp) encoding Neo was ligated (T4 DNA ligase; Thermo Scientific) with the digested vector 11 (6 141 bp), thereby incorporating the neomycin resistance gene into the pBABE-gateway 12 retroviral vector. pBABE-neo-gateway-ERBB2 was subsequently constructed by Gateway 13 cloning [1] (Addgene). pBABE-puro-gateway-ERBB3 plasmid was constructed by a LR-14 Gateway recombination reaction between the wild-type pDONR221-ERBB3 [2] and the 15 pBABE-puro-gateway using LR clonase II mix (Invitrogen). ..



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    93
    Addgene inc pbabe neo gateway erbb2
    Pbabe Neo Gateway Erbb2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbabe+neo+gateway+erbb2/pBABE-neo+(Plasmid+%231767)/pmc11245391__41388_2024_3070_MOESM1_ESM-2-35-44
    Average 93 stars, based on 1 article reviews
    pbabe neo gateway erbb2 - by Bioz Stars, 2026-09
    93/100 stars
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